Although soybean rust was not detected in Wisconsin n 2005, Plant Disease Diagnostics Clinic (PDDC) staff spent a substantial portion of their time this past growing season examining soybean samples for evidence of soybean rust. The basic strategy for monitoring samples for soybean rust at the PDDC uses an initial visual and microscopic examination of putative rust samples, with a follow-up where appropriate with a more sensitive technique called polymerase chain reaction (PCR).